Diaplate™MD6-70
Couldthisbeyournewcrystallizationplate?
ThedisposableDiaplateisa96wellmicro-dialysisplateforverysmallvolumesofuptoamaximumof3.2µL.ThesamplesolutionisPipettedontoeachofthe96membraneswithupto0.35mLuniquedialysissolutionsinthewellsusedforexchange.Dialysisisrecommendedfor2-3hoursbeforethedialysisbufferischangedandleftforanother2-3hourstoachieveover90%exchange.Furtherdialysisstepscanbecarriedoutasrequired.Incubationonarockingtableforspeedandamoisturechamberforfullhumidificationcanbeappliedtotheexperimentifdesired.
TheDiaplate™kitincludes:Diaplatewith200MicronPressureAdhesiveSpacer,200MicronUVPCoverFilm,engravedUVProtectiveCoverandasealingPaddle.
OwingtothehighpositionofthemembraneandtheUVtransparentcover,samplesonDiaplatecanbeobservedbymicroscopy,fluorimetry,turbidimetryandlightscattering.Diaplateisthereforeidealforcrystallizationbydialysisbychangingionicstrength,orsimplyofferingdifferentkineticstovapourdiffusion.
FeaturesofDiaplate:
- Simplehandling,readytouse,easysamplerecovery
- Completekit,noadditionalmaterialsrequired
- Pipettingcanbeperformedmanuallyorusingarobot
- Idealforthedialysisofupto3.2µLproteinsolution
- Nocrosscontaminationorleakagebetweenwellsguaranteed
- 96individualregeneratedcellulosemembranes;sulphurandheavymetalfree
- Molecularweightcutoffof10,000Daltons
- Proteincrystallizationapplications
- Lowproteinbindingandhighproteinretention
- Dialysiscompletein2-4hours
- Gentleandefficientdialysiswithoutdisturbingvaluableproteincomplexes
- Highthroughput;simultaneousprocessingof96experiments
- Reliableanduniform,stableconstruction
- Singleuse-disposabledevice;nocleaningrequired