Top96CrystallisationScreenMD18-07T/07
Features:
- ThelatestscreenfromJanetNewman&Colleagues
- 96ofthemostproductiveconditionsintheProteinDataBank
- Identicaltoatleast17%ofallPDBentries
- Highlysimilartoatleast40%ofallPDBentries
- Non-redundantsamplingofsuccessfulconditions
EverwonderwhatarethemostcommonlyoccurringconditionsreportedintheProteinDataBank?TOP96providesjustthat-asetof96conditionsthatoccurwithhighest,non-redundantfrequencyamongstallPDBdeposits.
Commercialcrystallizationscreensoffermorethan15000crystallizationconditionsforreadyscreeningofnewcrystallizationtargets.However,notallconditionsarewell-representedintheProteinDataBankandsomehavebeenfarmoresuccessfulthanothers.TOP96wasgeneratedbycreatingtwodatabases-onefornon-redundantcommerciallyavailablecrystallizationconditionsandoneforuniquepairsofproteinsequenceswithcrystallizationconditionsreportedinREMARK280ofeachPDBentry.Bothstrictand"fuzzy"overlapsbetweenthetwosetswerecalculatedandthemostfrequentlyreportedcrystallizationconditionsidentified.
Theresult?Themostproductive96crystallizationconditionsthatcanbeextractedfromtheentirePDBarchiveandthebestplacetostartyourcrystallizationscreening.
Citations:
- FazioVJ,PeatTS&NewmanJ(2014).AdrunkensearchinCrystallizationSpace.ActaCryst.F70:1303