PurePEGS-96CrystalScreenMD18-05T/05

Features:

• 96IndividualCrystallizationConditions
  
• UltraPurePEGs-USPGradePolydisperse
  
• PEGCocktail-5PEGsineachcondition
  
• pHmeasuredandrecordedforboththestockbuffersandfinalcrystallizationconditions

ThePurePEGsCrystallizationScreenfeaturesconditionsthateachcontainamixtureofpurifiedpolyethyleneglycols,rangingfrom300MWto8000MW.Atatotalconcentrationof22.5%PEGcombinedwithvarioussaltsandbuffers,theconditionsaredesignedtospanawidecrosssectionofPEGcrystallizationspace.

ManysuccessfulcrystallizationconditionscomprisecombinationsofPEGsandsaltsadjustedtoaparticularpH.However,thenecessitytosampleabroadrangeofPEGprecipitantsduringinitialsparsematrixscreeningrenderscomprehensivePEGscreeningatthisstagehighlychallenging.Interestingly,thereisevidencetosuggestthatproteinconcentrationconditionsidentifiedusingaparticularPEGcanberepeatedwhenusingmixturesofPEGsasasingleprecipitant.Consequently,amorerationalscreeningstrategyshouldbetousemixturesofPEGsratherthanconditionscontainingindividualPEGs.InVaporDiffusionexperiments,thisapproachsignificantlyminimizesthenumberofconditionstobesampledandthevolumeofproteinnecessarytoexplorePEGcrystallizationspace.

Citations:

1. J.Newmanetal.ActaCrystallogrD.61,1426(2005)
2. R.Pageetal.ActaCrystallogD.59,1028(2003)
3. J.W.Woohetal.ActaCrystallogD.59,769(2003)
4. J.T.Bukrinskyetal.JApplCryst.34,533(2001)