MCSG1CrystallisationScreenMD18-01T/01
Features
- 384non-redundantCrystallizationconditions
- Derivedfromadecadeoftrialsofmorethan40,000diverseproteinsattheMidwestCenterforStructuralGenomics
- Broadcoverageofcrystallizationspace
- Derivedfromcrystallizationoffrombothprokaryoticandeukaryoticorganisms
TheMCSGSuitehasbeendevelopedattheMidwestCenterforStructuralGenomics.AspartoftheProteinStructureInitiative,MCSGhasbeeninstrumentalinthedevelopmentofrapid,integratedandcost-effectivemethodsforproteinstructuredeterminationviaX-raycrystallography.
ThecompleteMCSGsuite(384conditions)comprisesof4screensof96uniqueconditionsthatareavailableeitherasdeep-wellblocks(1.7mLperwell)ortubes(10ml).Conditions1-288areorganizedinorderofdecreasingcrystalproductivityinscreensMCSG-1,MCSG-2andMCSG-3.ThusMCSG-1:A1conditionhasyieldedthegreatestnumberofuniquecrystalsforalltargetsscreenedwiththesuitepredecessors.TheconditionsthatcompriseMCSG-4havebeenrationallyselectedtosystematicallysampleregionsofcrystallizationspaceforproteinsrequiringadditionalscreening.
Theendresultisoneofthebest,first-passscreeningsuitespossIBLe,with:
- Data-drivenselectionindividualconditionsensuringconditionswithhighproductivitypotentialwerenotaccidentallyexcluded
- Superiororganization
- ThebroadestcoverageofcrystallizationspacewithinareasonablenumberofstartingconditionsformaximalefficiencywhilemaintainingapplicABIlityformoreuniqueanddifficulttargets.
