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Molecular Dimensions/Crystallophore No 1 (single vial , 0.6mg)/MD2-80/1 Ea
CrystallophoreNo1(singlevial,0.6mg)MD2-80
PolyvalanCrystallophoreNo1(patentpending)isaluminescentTerbiumcomplexthatisaneffectivenucleantandprovidessignificantisomorphousandanomalousphasingpower.ReadmoreinfomationabouthowCrystallophoreNo1workshere
BREAKINGNEWS:ThefirstnovelstructuresolvedusingCrystallophoreNo1,BacteriophageT5distaltailprotein(PDBID:5MF2)hasjustbeenreleasedintheProteinDataBank.Couldyoursbethenext?Tellusaboutit!
FORMING
CrystallophoreNo1actsasanucleatingagentandimprovescrystalqualityofpre-existinghits.AsshownrightforT5phagewithandwithoutCrystallophoreNo1(topright).
FINDING
CrystallophoreNo1luminescesstronglyinthevisIBLeregionunderUVexcitationmakingdetectingcrystalsindropsandloopseasy.Seemiddleright.
PHASING
TerbiumisalanthanideandthereforehassignificantpotentialforuseasananomalousscattererandintrADItionalisomorphousreplacement.WhenCrystallophoreNo1hasbeenusedasanucleant,itisoftenfoundboundtotheproteinthusprovidinghighqualityphasingpower.
NEXTSTEPS
Tohelpyoubuildthestructureyoucandownloadthepdborcifformatco-ordinatefilesfortheCrystallophoreNo1complex.KindlyprovidedbyPolyvalan.
READALLABOUTIT
ThedevelopmentworkleadingtoCrystallophoreNo1hasnowbeenpublishedin:
Engilberge,S.,Riobé,F.,DiPietro,S.,Lassalle,L.,Coquelle,N.,Arnaud,C.-A.,Pitrat,D.,Mulatier,J.-C.,Madern,D.,Breyton,C.,Maury,O.andGirard,E.Crystallophore:aversatilelanthanidecomplexforproteincrystallographycombiningnucleatingeffect,phasingpropertiesandluminescence.ChemicalScience8:5909-5917(2017).
(bottomright)P.horikoshiiProtease1electrondensityfromSADphasingusingtheTerbiumLIIIabsorptionedge.Crystalgrownin10mMandsubsequentlysoakedin100mMcrystallophoreNo1.
Eachvialccontains0.6mgofthecomplex,enoughtosetup696-wellplatesofyourtargetproteinincomplexwith10mMCrystallophoreNo1(recommendedconcentration).Shippedatroomtemperature.Thedrypowderwillkeepforupto1yearinasealedtubeatroomtemperatureindryconditions.SolutionsofCrystallophoreNo1canbekeptforupto2monthsinasealedtube.Solutionsofthecompoundmixedwithyourtargetproteinshouldbeusedimmediately.