CryoProtX™MD1-61
Thisbrand-newinnovativeCryoKithasbeendevelopedandformulatedbyDr.EnricoSturaandLauraVeraatCEASaclay,France.Thiskitisdesignedforthecryoprotectionofproteincrystalsandisforuseafterthecrystalshavebeengrown.
Getmorefromyourcrystalswiththisnew,multi-componentcryoprotectantkitforproteincrystals.
- Preservethehighestdiffractionpotentialofcrystals.
- Ready-mademixtures.
- IncorporatespHscreening.
- Crystalslesslikelytocrackordissolve.
- Idealforheavyatom,ligandorbacksoaks.
- Screenwithlow-affinity,low-solubilityinhibitors.
- Customisationoptions-canbecustomisedtomatchmostcurrentprojects.
StockreagentsofthecomponentsofthiskitandcanalsobepurchasedfromMolecularDimensions.
Vera,Laura,andEnricoA.Stura."Strategiesforproteincryocrystallography."CrystalGrowth&Design14.2(2013):427-435.
"Picturethisscenario:mondayafternoon,thedewarisabouttoleavethelabwithcrystalsforsynchrotronmeasurement;youdoafinalinspectiontoyourcrystallizationplateseagerlyhopingforcrystalsofyounewprojectand...voilá!!!Oneconditionhasfourbeautifulcrystals!!!Istherestilltimetofreezethemandsendthemwiththisshipment???Istheconditioncryo???Willtheydiffract???SinceweboughttheCryoProtXcryokit,someofthequestionsabovewereself-answered:yes,therewillbetime(CryoProtXwillsaveyoutime!!),yes,itwillbecryo(CryoProtXalwayshasaconditionthatyourcrystalswilllike!!!),yes,itwilldiffract(inourhands,CryoProtXyieldedatleastthreenewstructuresthatweredenovophasedusingdatacollectedfromcrystalscryoprotectedwithCryoProtX!!).Weareveryverypleasedwiththiskitandwestronglyrecommendit,notonlyasalastresort(orlastminute)tool,butasanimportantstepinthe"fromgenetostructure"pipelineofanyMacromolecularCrystallographyLaboratory.
Rosario,A.R.andBrito,J.A.,etal.(2015),ActaF71,477-482
Sena,F.V.andBrito,J.A.,etal.(2015),Molec.Microbiol.98(2),272-288
BritoJ.A.,etal.(2016)twosubmittedpapersandoneinpreparation"
JoseArturBrito,ITQB-UNL,Portugal
