分子尺寸/蛋白质稳定性组合试剂盒/MD1-103/1 Ea-免疫在线蚂蚁淘旗下平台

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商品详细分子尺寸/蛋白质稳定性组合试剂盒/MD1-103/1 Ea

分子尺寸/蛋白质稳定性组合试剂盒/MD1-103/1 Ea

商品编号： MD1-103

品牌： moleculardimensions

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：其他试剂

公司分类： Other_reagents

联系Q Q： 3392242852

电话号码： 4000-520-616

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商品介绍

TheProteinStABIlityComboKitMD1-103

Thermalshiftassaysprovideinformationontheeffectvariousenvironmentalfactorshaveonthethermaldenaturationofaprotein.Thermaldenaturationisrelatedtoproteinstabilityandthereforecrystallizability.ThemostcommonthermalshiftassayisThermoFluor^®,whichmeasuresincreasingfluorescenceinducedbySYPRO^®Orangebindingtotheexposedproteinhydrophobiccore.Buttherearemanyothersdependingontherequirementsofyoursample.

Ourproteinstabilityscreensarenowavailableinonepackageatadiscountofmorethan20%comparedtobuyingthemseparately(orapproximately4kitsforthepriceof3).

TheDurhamScreens

DevelopedbyEmilyCardewandEhmkePohlattheUniversityofDurham,thesekitsprovidegreaterdepthofanalysisoftheeffectofindividualbuffers,pHandsalts.TheDurhamscreenisdesignedtoworkwithNAMI,aGUI-basedprogramtoaidinterpretationofthermalshiftdata.

TheDurhampHScreencontainsdeconvolutesbuffermoleculeandpHeffectsforaboradrangeofmolecule(28differentbuffers)andpH(4-11).

TheDurhamSaltScreenusesmorethan30differentsalts,includingarangeoflanathanides.Itallowdiscriminationbetweendifferentanionsandcationsaswellasrevealingtheeffectsofionicstrength.

ReadmoreaboutTheDurhamScreensin:Grøftehauge,MK,Hajizadeh,NR,Swann,MJandPohl,E.Protein-ligandinteractionsinvestigatedbythermalshiftassays(TSA)anddualpolarizationinterferometry(DPI).ActaCrystD71:36-44(2015).

TheRUBICScreens

InventedbyStaphaneBoivinandRobMeijersattheSamplePerparationandCharacterisationfacilityatEMBLHamburg.TheRubicsscreenconsistsoftwokits:

TheRUBICBufferScreendeconstructsthebuffercomponentstoallowarationalinterpretationanddeconvolutionofpH,buffermoleculeandionicstrengtheffects.

TheRUBICAdditiveScreenanalyzestheeffectonstabilityofvariouscommonadditivesincludingions,detergents,sugars,aminoacids,imidazole,cryoprotectantsandreducingagents.

TheRUBICScreensaredescribedin:BoivinS,KozakS,MeijersR.(2013)OptimizationofproteinpurificationandcharacterizationusingThermofluorscreens.ProteinExprPurif.91(2):192-206.

Reference:NewmanJ.(2004)Novelbuffersystemsformacromolecularcrystallization.ActaCrystallogrDBiolCrystallogr.60:610-2.

Storageconditions:

TheDurhampHScreenispresentedas96x0.5mLconditionsinadeep-wellblock.Theyarepreservativefreeanditisrecommendedthattheyarestoredat4°C,howevertheycanbeusedatroomtemperatureforscreenset-up.

RUBICBufferscreenisfreeofpreservatives.ShippingatRoomTemperature.Short-termStorageat4˚C.Itisrecommendedthatusersprefillplatesandstorethemat-20˚C.

ThermoFluorisaregisteredtrademarkofJohnsonandJohnson.SYPROisaregisteredtrademarkofLifeTechnologies.

品牌介绍

Molecular Dimensions 来自 digital.library.unt.edu  喜欢 0 阅读量： 1 作者： Moss, H. H 摘要： This study attempts to calculate the areas of the cross section and diameter of the CH3 group, COOH group, the length of the carbon chain, and the longtiudinal distance between carbon atoms for each of the three acids. It also attempts to develop a method of measuring the effective diameter of molecules of gases. Equations for these terms as given by MacDougall and experiments were performed and the data applied to the equation. 关键词： carbon chain carbon atoms diameter of molecules of gases

公司介绍

Molecular Dimensions 来自 digital.library.unt.edu  喜欢 0 阅读量： 1 作者： Moss, H. H

品牌分类

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