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Molecular Dimensions/Top 96 Crystallisation Screen/TOP96/TOP96-10ML
- ThelatestscreenfromJanetNewman&Colleagues
- 96ofthemostproductiveconditionsintheProteinDataBank
- Identicaltoatleast17%ofallPDBentries
- Highlysimilartoatleast40%ofallPDBentries
- Non-redundantsamplingofsuccessfulconditions
EverwonderwhatarethemostcommonlyoccurringconditionsreportedintheProteinDataBank?TOP96providesjustthat-asetof96conditionsthatoccurwithhighest,non-redundantfrequencyamongstallPDBdeposits.Commercialcrystallizationscreensoffermorethan15000crystallizationconditionsforreadyscreeningofnewcrystallizationtargets.However,notallconditionsarewell-representedintheProteinDataBankandsomehavebeenfarmoresuccessfulthanothers.TOP96wasgeneratedbycreatingtwodatabases-onefornon-redundantcommerciallyavailablecrystallizationconditionsandoneforuniquepairsofproteinsequenceswithcrystallizationconditionsreportedinREMARK280ofeachPDBentry.Bothstrictand"fuzzy"overlapsbetweenthetwosetswerecalculatedandthemostfrequentlyreportedcrystallizationconditionsidentified.Theresult?Themostproductive96crystallizationconditionsthatcanbeextractedfromtheentirePDBarchiveandthebestplacetostartyourcrystallizationscreening.Citations:
- FazioVJ,PeatTS&NewmanJ(2014).AdrunkensearchinCrystallizationSpace.ActaCryst.F70:1303
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